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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/12/2015 |
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Data da última atualização: |
09/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARMO, C. D. do; SILVA, M. S. da; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de. |
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Afiliação: |
CÁTIA DIAS DO CARMO, UFRB; MAIANE SUZARTE DA SILVA, UFRB; GILMARA ALVARENGA FACHARDO OLIVEIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Molecular-assisted selection for resistance to cassava mosaic disease in Manihot esculenta Crantz. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Scientia Agricola, v.72, n.6, p.520-527, November/December, 2015. |
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ISSN: |
0103-9016 |
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Idioma: |
Português |
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Conteúdo: |
The geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was not found in M. esculenta. In contrast, the CMD2 gene was found in 5, 4 and 5 % of cassava accessions, with flanking markers NS169+RME1, NS158+RME1 and SSRY28+RME1, respectively. Only seven accessions presented all markers linked to the CMD resistance. The DAPC of the seven accessions along with 17 elite cassava varieties led to the formation of three divergent clusters. Potential sources of resistance to CMD were divided into two groups, while the elite varieties were distributed into three groups. The low estimates of the genomic relationship (ranging from -0.167 to 0.681 with an average of 0.076) contributed to the success in identifying contrasting genotypes. The use of MAS in countries where CMD is a quarantine disease constitutes a successful strategy not only for identifying the resistant accessions but also for determining the promising crosses. MenosThe geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was not found in M. esculenta. In contrast, the CMD2 gene was found in 5, 4 and 5 % of cassava accessions, with flanking markers NS169+RME1, NS158+RME1 and SSRY28+RME1, respectively. Only seven accessions presented all markers linked to the CMD resistance. The DAPC of the seven accessions along with 17 elite cassava varieties led to the formation of three divergent clusters. Potential sources of resistance to CMD were divided into two groups, while the elite varieties were distributed into three groups. The low estimates of the genomic relationship (ranging from -0.167 to 0.681 with an average of 0.076) contributed to the success in identifying contrasting genotypes. The use of MAS in countries where CMD is a quarantine disease ... Mostrar Tudo |
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Palavras-Chave: |
Plant diseases. |
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Thesagro: |
Doença de planta; Mandioca; Vírus. |
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Thesaurus Nal: |
Breeding; Cassava. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02309naa a2200241 a 4500
001 2032242
005 2016-03-09
008 2015 bl uuuu u00u1 u #d
022 $a0103-9016
100 1 $aCARMO, C. D. do
245 $aMolecular-assisted selection for resistance to cassava mosaic disease in Manihot esculenta Crantz.
260 $c2015
520 $aThe geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was not found in M. esculenta. In contrast, the CMD2 gene was found in 5, 4 and 5 % of cassava accessions, with flanking markers NS169+RME1, NS158+RME1 and SSRY28+RME1, respectively. Only seven accessions presented all markers linked to the CMD resistance. The DAPC of the seven accessions along with 17 elite cassava varieties led to the formation of three divergent clusters. Potential sources of resistance to CMD were divided into two groups, while the elite varieties were distributed into three groups. The low estimates of the genomic relationship (ranging from -0.167 to 0.681 with an average of 0.076) contributed to the success in identifying contrasting genotypes. The use of MAS in countries where CMD is a quarantine disease constitutes a successful strategy not only for identifying the resistant accessions but also for determining the promising crosses.
650 $aBreeding
650 $aCassava
650 $aDoença de planta
650 $aMandioca
650 $aVírus
653 $aPlant diseases
700 1 $aSILVA, M. S. da
700 1 $aOLIVEIRA, G. A. F.
700 1 $aOLIVEIRA, E. J. de
773 $tScientia Agricola$gv.72, n.6, p.520-527, November/December, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 66 | |
| 9. |  | OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de. Desenvolvimento de SNP (Single-Nucleotide Polymorphism) para estudos de variação genética e estabelecimento de coleção nuclear em mamoeiro (Carica papayaL.). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | MEDEIROS, R. D.; ARAÚJO, W. F.; OLIVEIRA, G. A.; ZILLI, J. E. Irrigação na cultura do feijão-caupi. In: WORKSHOP SOBRE A CULTURA DO FEIJÃO-CAUPI EM RORAIMA, 2007, Boa Vista, RR. Anais... Boa Vista, RR: Embrapa Roraima, 2007. (Embrapa Roraima. Documentos, 4).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Roraima. |
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| 18. | | COSTA, J. L. C.; OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; SILVA, A. dos S.; YAMAGISHI, M. E. B. Minissatélites: novas ferramentas moleculares para o mamoeiro. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 915| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | COSTA, J. L.; OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; CARVALHO, F. M. de; DANTAS, J. L. L. Otimização de locos de microssatélites para diferentes espécies de Passiflora. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 4., 2010, Cruz das Almas. [Anais...] Cruz das Almas: Embrapa Mandioca e Fruticultura, 2010. (Embrapa Mandioca e Fruticultura. Documentos, 190). 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 66 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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